• Home
• About Us
• Programs/Activities
• Publications
• Resources
• Media Center
• Altweb
• Contact Us
• Make a Gift

Curriculum Vitae - Pamela J. Lein, Ph.D.

PERSONAL DATA:

University of California at Davis
School of Veterinary Medicine
Department of Molecular Biosciences
1120 Haring Hall, One Shields Ave
Davis, CA 95616
Phone: 530-752-1970
Fax: 530-752-4698
Email: pjlein@ucdavis.edu

EDUCATION AND TRAINING:

PROFESSIONAL EXPERIENCE:

Current:

Previous:

PROFESSIONAL ACTIVITIES:

Society Membership:

• Society of Toxicology (SOT)
• Society for Neuroscience (SFN)
• American Society of Cell Biology (ASCB)
• American Association for the Advancement of Science (AAAS)

Participation on Advisory Panels:

Program or Project Development

Consultantships:

Editorial Activities:

Peer Review Activities, Journals:

• Brain Research
• Journal of Biological Inorganic Chemistry
• Journal of Cell Biology
• Journal of Neurobiology
• Journal of Neurochemistry
• Journal of Neuroscience
• Journal of Neuroscience Methods
• Journal of Neurochemistry
• Neurotoxicology
• Toxicology Letters
• Toxicological Sciences

Editorial Board Membership:

• Current Neurobiology, Associate Editor, 2009-2012
• NeuroToxicology, Editorial Board, 2007-2009; Associate Editor, 2009-2012
• Toxicology and Applied Pharmacology, Editorial Board, 2007-2011, Associate Editor 2012-2014
• Toxicological Sciences, Editorial Board, 2011 - 2014
• Journal of Toxicology, Editorial Board, 2007-2010
• Toxicology Letters, Editorial Board, 2001-2004

Ad Hoc Review of Proposals:

• Pilot project review, UC Davis Alzheimer’s Disease Center, University of California, Davis, CA, April 2012
• Pilot project review, NIEHS Center for Environmental Health, Columbia University, New York, NY, April 2012
• Ad hoc, Study section, NIH ZRG1 IFCN-A02, Neurotoxicology Special Emphasis Panel, March 2012
• Standing Member, Study section, NIH, Environmental Health Sciences Review Committee (EHSRC), the NIEHS chartered review committee, Sept 2011 - June 2015
• Ad hoc, Study section, NIH EHSRC, Environmental Health Sciences Review Committee, The NIEHS chartered review committee, Nov 2010, April 2011
• Standing Member, Study section, NIH NAL, Neurotoxicology and Alcoholism, Oct 2007 – Sept 2011
• Ad hoc, Study section, NIH ZRG1 BDCN-N, Neurotransmitters and Neuroplasticity, Apr 2007
• Ad hoc, Study section, NIH NAL, Neurotoxicology and Alcoholism, Oct 2006
• Ad hoc, Study section, NIH NIGMS, Minority Biomedical Research Support (MBRS) Support of Continuous Research Excellence (SCORE) in Neuroscience, 2006
• Ad hoc, Study section, NIH NIEHS, Conference Grant Applications (R13), 2002 - 2005
• Chair, NIH NIEHS, Special Emphasis Panel, 2002
• Member, NSF Integrative Organismal Biology/Developmental Systems Cluster, 2005-2007
• Ad hoc, NSF, Developmental Neuroscience Program, 1999-2002, 2004-2006
• Ad hoc, National Science Foundation, Research Experience for Undergraduates Program, 2001
• The Davidson Institute for Talent Development, Davidson Fellows Submission Review, 2004
• Center for Alternatives to Animal Testing (CAAT), 2001-2004, 2009 - 2011
• Johns Hopkins Center in Urban Environmental Health, 2001, 2004, 2009

AWARDS & HONORS:

• NSF ILI grant, Incorporation of imaging techniques into undergraduate cell and molecular laboratories (DUE-9850762), 1998-1999
• NSF ILI grant, Laboratory revision in genetics and improvements in cell and molecular courses (DUE-9750452), 1997-1999
• Howard Hughes Medical Institute Program for Undergraduate Science Education, Co-P.I., 1996-1999
• Dean's Grant, Arts and Sciences, Canisius College, 1995, 1996
• Grass Fellowship Exchange, The Grass Foundation, 1994-1997
• National Science Foundation Research Opportunity Award, 1994
• NIH NCI National Research Service Award, Cell biology of human TILs engrafted into SCID mice (1 F32 CA09177-01), 1991-1994
• March of Dimes Predoctoral Fellowship, 1987-1990

TEACHING:

Advisees:

Project Scientist/Staff Scientist Mentor

• Ana Cristina Goncalves Grodzki, 2010 – present
• Dongren Yang, 2007 - 2012

Postdoctoral advisor - University of California, Davis

• Jessica Spradley, 2012 – present
• Paula Goines, 2011 – present
• Chris Banks, 2010 – 2011
• Ana Cristina Goncalves Grodzki, 2009 – 2010
• Oregon Health & Science University
• Lauren Courter, 2007 – 2009
• Veronica Ledoux, 2007 – 2008
• Dongren Yang, 2003 – 2006
• Johns Hopkins University
• Xin Guo, 2002 -2003

Ph.D. Thesis Mentor - University of California, Davis

• Christopher Barnhart, 2010 – present
• Hao Chen, 2010 – present
• Marianna Stamou, 2010 – present
• Johns Hopkins University
• Hui-Ling Chen, Ph.D., Neuroscience, 2003
• Angela Howard, Ph.D., Environmental Health Sciences, 2003

Ph.D. Thesis Committee Member - University of California, Davis

• Myka Estes, Neuroscience, 2012 - present
• Carly Moore, Pharmacology and Toxicology, 2011-present
• Yassaman Niknam, Pharmacology and Toxicology, 2011- present
• Shannon Murphy, Pharmacology and Toxicology, 2009 – present
• Claire Koenig, Pharmacology and Toxicology, 2012

Ph.D. Thesis Committee Member - Oregon Health & Science University

• Lavakumar Ranganathan, Ph.D., Biomedical Engineering, 2007
• Norah Verbout, Ph.D., Physiology and Pharmacology, 2008
• Linda Ruggiero, Ph.D., Neuroscience Graduate Program, 2008
• Jill Wentzel, Ph.D., Neuroscience Graduate Program, 2010
• Christina Lorentz, Ph.D., Physiology and Pharmacology, 2010
• SUNY at Buffalo
• Eugene Gonsiorek, Ph.D., Pharmacology and Toxicology, 2006
• Johns Hopkins University
• Anne Sullivan, Ph.D., Environmental Health Sciences, 2005
• Lilian Morena, Ph.D., Environmental Health Sciences, 2003

M.S. Thesis Mentor - Johns Hopkins University

• Rosemary Schuh, M.Sc., Environmental Health Sciences, 2001

Graduate Rotation Student Advisor -

University of California, Davis

• Anna Kruetz, Neuroscience, Fall 2012
• Frances Wilburn, Pharmacology and Toxicology, Fall 2012
• Seiya Kitamura, Pharmacology and Toxicology, Winter 2011
• Destanie Cummings, Microbiology and Immunology, Fall 2011
• Stephen Vito, Pharmacology and Toxicology, Fall 2011
• Lindsey Curley, Pharmacology and Toxicology, Fall 2011
• Esther Shin, Pharmacology and Toxicology, Winter 2011
• Sam Chung, Pharmacology and Toxicology, Fall 2010
• Anahita Hamidi, Neuroscience, Fall 2010
• Monica Delgado, Microbiology and Immunology, Winter 2010
• Yassaman Niknam, Pharmacology and Toxicology, Winter 2010
• Emily Mills, Molecular Microbiology and Immunology, Fall/Winter 2009/2010
• Candace Burke, Molecular Microbiology and Immunology, Fall/Winter 2009/2010
• Hao Chen, Pharmacology and Toxicology, Fall 2009
• Shannon Conley, Pharmacology and Toxicology, Fall 2009
• Christopher Barnhart, Pharmacology and Toxicology, Fall 2009

Oregon Health & Science University

• Cadence True, Neuroscience Graduate Program, Spring 2008
• Tracie Nettleton, Program in Molecular and Cellular Biology, Winter/Spring 2008
• Trevor Levin, Program in Molecular and Cellular Biology, Spring 2007
• Kalmia Smith, Program in Molecular and Cellular Biology, Winter 2006
• Kristine Alexander, Program in Molecular and Cellular Biology, Fall 2005
• Nicholas Murchison, Program in Molecular and Cellular Biology, Spring 2005
• Joyce Wondolowski, Neuroscience Graduate Program, Spring 2004
• Edwin Reese, Neuroscience Graduate Program, Fall/Winter/Spring 2003/2004

Member Qualifying Exam Committee -

University of California, Davis
*Chair of examination committee

• *Emir Leon, Pharmacology and Toxicology, 2012
• Yassaman Niknam, Pharmacology and Toxicology, 2011
• Yezi Zhu, Pharmacology and Toxicology, 2011
• Myka Estes, Neuroscience, 2011
• Candace Burke, Immunology, 2011
• Jack Hsiang, Pharmacology and Toxicology, 2010
• Charity Onore, Immunology, 2010
• Claire Koenig, Pharmacology and Toxicology, 2009, 2010
• Shannon Murphy, Pharmacology and Toxicology, 2009

Oregon Health & Science University
*Chair of examination committee

• Amy Bruestle, Neuroscience, 2008
• *Elad Kaufman, Physiology and Pharmacology, 2008
• *Kalmia Buels, Physiology and Pharmacology, 2007
• Jeffrey Iliff, Physiology and Pharmacology, 2007
• Meghan Lindauer, Cell and Developmental Biology, 2007
• *Jessica Martin, Neuroscience, 2007
• Nicholas Vyleta, Neuroscience, 2007
• Chang Liu, Cell and Developmental Biology, 2006
• Christina Lorentz, Physiology and Pharmacology, 2006
• Jill Wetzel, Neuroscience, 2006

Johns Hopkins University Preliminary Oral Participation

• Mehdi Saeed Hazari, Environmental Health Science/Physiology, April 2003
• Benjamas Chuaychoo, Environmental Health Science /Physiology, November 2002
• Britt Luccy, Environmental Health Science /Toxicology, October 2002
• Louis Stein, Environmental Health Science /Physiology, June 2002
• Lilana Moreno, Environmental Health Science /Physiology, November 2001
• Anne Sullivan, Environmental Health Science /Toxicology, October 2001
• Rosemary Schuh, Environmental Health Science /Toxicology, April 2001
• Angela Howard, Environmental Health Science /Toxicology, November 2000
• Lori Sturtz, Environmental Health Science /Toxicology, November 2000
• Minerva Gomez, Environmental Health Science /Toxicology, January 2000
• Deborah Segal, Environmental Health Science /Toxicology, November 1999

Johns Hopkins University Final Oral Participation

• Mehdi Saeed Hazari, Environmental Health Science/Physiology, June 2003
• Rebeca Burdeinick-Kerr, Molecular Microbiology and Immunology, February 2003
• Sara Volk, Biochemistry, October 2002
• Ching Ging Ng, Molecular Microbiology and Immunology, September 2002
• Jessica Darman, Molecular Microbiology and Immunology, May 2001 Angela Howard, Environmental Health Science/Toxicology, December 2000

Undergraduate Summer Research Advisor -

University of California, Davis

• Rachel Shaffer, Yale University, NIEHS Undergraduate Research Program, 2011
• Linley Mangini, UC Davis, NIEHS Undergraduate Research Program, 2010
• Sarah Valez, Colorado College, NIEHS Undergraduate Research Program, 2009

Oregon Health & Science University

• Holly Lauridsen, Brown University, CROET Undergrad Summer Research Program, 2008
• Abby Engebose, Beloit College, CROET Undergrad Summer Research Program, 2006, 2007
• Levanna Goldberg, Lincoln H.S., CROET Undergrad Summer Research Program, 2005
• Stacy Brendtro, Middlebury College, CROET Undergrad Summer Research Program, 2004

Johns Hopkins University

• Jennie Huang, Johns Hopkins University, 2002
• Camille Henson, Hampton University, 2001
• Delise Charles, Morgan State University, 2001
• Kroshona Tabb, Alabama State University, 2001
• Robert Bucelli, Canisius College, 2000

Undergraduate Honors Thesis Advisor

University of California, Davis

• Linley Mangini, 2010-2011; 2011-2012

Canisius College, Buffalo, NY

• Danielle Dorsaneo, Hiroko Nagasawa, Laurie Robak, 1999
• Patrick J. Gallagher, Christopher Lang, Rachelle Toman, 1998
• Ann Marie Hedges, 1997
• Jeffrey Amodeo, 1996

Classroom Instruction

University of California, Davis

Neurotoxicology, VMB/ETX234, Spring 2010, 2012, Instructor of Record
Neurotoxicology, VMB/ETX235, Spring 2010, 2012
- Principles of cellular neurobiology (two 90 minute lectures)
- Introduction to neurotoxicology (90 minute lecture)
- Animal models (90 minute lecture)
- Axonopathies (90 minute lecture)
Principles of Pharmacology and Toxicology, PTX 201, Fall quarter, 2010-2011
- How to review a scientific article (one 50 minute lecture)
Principles of Pharmacology and Toxicology, PTX 203, Spring quarter, 2009-2012
- Autonomic innervation of the airways and asthma (two 50 minute lectures)
Veterinary Toxicology, VMD414C, Spring quarter, 2009-2011
- Metals (two 50 minute lectures)
Neuro Block, VET404, Spring quarter 2012
- ANS Pharmacology (two 50 minute lectures)
- ANS Toxicology (two 50 minute lectures)
M.I.N.D. Institute, Autism Research Training Program
Neurochemistry/Psychopharmacology, Spring 2009, Summer 2011
- Overview of Neurochemistry (one 90 minute lecture)
Neurotoxicology, Summer 2009, Spring 2011
- Overview of Developmental Neurotoxicology (one 90 minute lecture)
- OPs as Environmental Risk Factors for Autism (one 90 minute lecture)

Oregon Health & Science University, 2003 – 2008

Cell and Molecular Neurobiology, NEUS 625, Course Coordinator
Cell and Molecular Neurobiology, NEUS 625, Lecturer
- Overview: Cells of the Nervous System
- Overview: Neurodevelopment
- Neuroglial Interactions
- Discussion of Programmed Cell Death
Systems Processes and Homeostasis, MSCI 613
- Pharmacology Tutorial IV – Drug Overdose, Faculty Facilitator
- Cholinergic Pharmacology Tutorial – Drug Action at NMJ, Faculty Facilitator
Organ Systems, CON 667, Lecturer
- Neural Plasticity
Advanced Topics in Developmental Neuroscience, CELL 615
- Axon specification in vitro: a model of neuronal morphogenesis in vivo?
- Target-dependent regulation of dendritic growth and plasticity

Oregon State University, 2009

Target Organ Toxicology, TOX 512, OP Module
- Pathophysiological effects of OPs on biological systems

Johns Hopkins University School of Public Health, 1999-2003

Fundamentals of Neurotoxicology, 187.661, Course Coordinator
Fundamentals of Neurotoxicology, 187.661, Lecturer
- Introduction and overview
- Neurotoxins and axonopathy
- Transmission toxicity
- Special issues: developmental neurotoxicity
Introduction to Physiology, 020.130
- Muscle Physiology
- Environmental Health Relevance of Nervous System Physiology
Principles of Toxicology, 187.610
- Case Studies in Toxicology: Persistent Organic Pollutants and Dioxins
- Case Studies in Toxicology: Neurotoxins
Environmental Health, 180.601
- Xenoestrogens - New Man-Made Threats
Fundamentals of Reproductive Biology, 120.620
- Endocrine Disruptors
Topics in Molecular Endocrinology, 120.621.01
- NeuroEndocrine Disruptors
Fundamentals of Human Physiology, 183.631
- Environmental Health Relevance of Nervous System Physiology
- Muscle Physiology
The Environment and Your Health, 570.303
- Risk Analysis
- Case Study: Endocrine Disruptors

Morgan State University

April 2001 Role of BMPs in Plasticity in the Adult Brain

SUNY at Buffalo School of Medicine and Biomedical Sciences, Buffalo, NY, 1993 - 1999

Cell Biology I, BSM 501, Cell Polarity
Cell Biology II, BSM 502
- Cell Polarity and Integrins
- Neuronal Polarity
- Cell Adhesion Molecules
Developmental Neurobiology, PMY 625, Adhesion Molecules in the Nervous System

Canisius College, 1993 – 1999

BIO 101, Introduction to Cellular/Subcellular Biology
BIO 102, Introduction to Organismal Biology
BIO 135, Environmental Biology
BIO 300, Research Techniques
BIO 352, Junior Seminar
BIO 360, Environmental Health
BIO 360L, Environmental Health Laboratory
BIO 400, Independent Study
BIO 401, Independent Research
BIO 423, Neuroscience I
BIO 423L, Neuroscience I laboratory
BIO 425, Cellular Neurobiology
BIO 425L, Cellular Neurobiology Laboratory
BIO 435, Developmental Neurobiology
BIO 430, Medicinal Botany
BIO 452, Senior Seminar
HHMI Integrated Science Course

Niagara University, Niagara Falls, NY, 1996 - 1999

BIO491, Bio-Analytical Techniques and Laboratory
- Cell culture and indirect immunofluorescence

Professional Courses, 1993 - 1999

PMY831, Dental Pharmacology, Nicotinic Blocking Agents, SUNYAB School of Dental Medicine
Professional training course, Development of Emergency Action Levels, West Valley Nuclear Services, Inc., West Valley, NY
PMY731, Basic Principles in Pharmacology, Teratogenic agents, SUNYAB School of Medicine

RESEARCH GRANT PARTICIPATION:

Active

R01 ES 016308 (Anger (Contact) and Lein, MPI) 06/01/08 – 04/30/13
NIH NIEHS
Biomarkers of Organophosphorus Pesticide-Induced Neurotoxicity.
The main objective of this project is to test the hypotheses that OP-induced neurobehavioral deficits in humans are dose-related and that biomarkers of oxidative stress and inflammation are better predictors of neurobehavioral deficits than cholinesterase inhibition.

R01 ES014901 (Lein(Contact) and Pessah, MPI) 12/01/08 – 11/30/13
NIH NIEHS
Molecular and Cellular Basis of PCB Developmental Neurotoxicity
The main objective of this grant is to test the hypothesis that non-coplanar PCBs disrupt neuronal connectivity via ryanodine receptor (RyR)-mediated mechanisms that modulate Ca2+-dependent signaling pathways linked to activity-dependent dendritic growth and plasticity.

T32 ES007059 (Lein) 07/01/2010 – 06/30/2013
NIH NIEHS
Advanced Training in Environmental Health Sciences
The objective of this grant is to support pre- and postdoctoral trainees in the area of environmental health sciences research.

R01 ES017425 (Lehmler (Contact), Lein, Pessah, MPI) 12/1/09-11/30/14
NIH/NIEHS
Enantioselective Metabolism Influences PCB Developmental Neurotoxicity.
The primary goal of the project is to explore whether the PCB atropisomers have differential potency and efficacy towards ryanodine receptors.

R01 ES017592 (Lein(Contact) and Fryer, MPI) 07/1/10-06/30/15
NIH NIEHS
Role of Macrophages in Organophosphorus Pesticide-Induced Airway Hyperreactivity.
The main goal is to test the hypothesis that airway macrophages mediate organophosphorus pesticide-induced airway hyperreactivity in non-atopic hosts.

P42 ES04699 (Hammock) 04/01/10 – 03/31/15
NIH NIEHS Superfund Research Program
Biomarkers of exposure to hazardous substances, Training Core C of the program project
The Training Core aims to produce doctoral level engineers and scientists with the interdisciplinary educational experience necessary to address complex research problems posed by hazardous waste sites. Role on project: PI of Training Core C

U54 NS079202 (Lein) 09/01/12 – 08/30/17
NIH NINDS CounterACT Program
Novel anticonvulsant and neuroprotective therapies for TETS and OP intoxication
The unifying goal of the UC Davis CounterACT Center of Excellence is to identify improved medical countermeasures for treating acute intoxication with seizure-inducing chemical threat agents.
Role on project: Center Director and PI of Project 2 of the Center

Completed

R21 NS072094 (Lein (Contact) and Rogawski, MPI) 10/01/2010 – 09/30/2012
NIH NINDS
Identification of novel therapeutic approaches to TETS and OP intoxication. The goal is to obtain preliminary data in support of our hypothesis that AMPA receptor (AMPA-R) antagonists and/or inhibitors of soluble epoxide hydrolases (sEHi) will significantly improve clinical management of acute TETS and parathion intoxication by extending the therapeutic window, enhancing neuro-protection and providing therapeutic efficacy against diverse chemical threat agents.

U01 NS 057993 (Ford) 09/27/06 – 05/31/11
NIH NINDS
Neuroprotective Roles for Neuregulins in Neurotoxin-Mediated Neuronal Injury. The major goal of this project is to test the hypothesis that neuregulins will be neuroprotective against CNS damage caused by OPs. (Role on project: PI on subcontract)

UC Davis M.I.N.D. Institute Pilot Project (Lein) 07/01/09 – 6/30/10
University of California, Davis
Potential Gene-Environment Interactions in ASD: Disruption of neuroligin-mediated synaptogenesis by organophosphorus pesticides. The major goal of this project is to test the hypothesis that organophosphorus pesticides interfere with neuroligin-mediated synapse formation in cultured hippocampal neurons.

R01 ES014601 (Fryer) 12/01/06–11/30/11
NIH/NIEHS
Asthma exacerbation by organophosphate pesticides. The goal of this project is to test the hypothesis that sensitized animals will be more sensitive to organophosphorus pesticides than non-sensitized animals and that organophosphorus pesticides will interact with eosinophils to further exacerbate airway hyperreactivity in this susceptible population. Role on grant: Co-Investigator

R01 NS046649 (Lein) 07/01/03- 04/30/08
NIH/NINDS
Retrograde dystrophic influence of IFN-gamma on neurons. The main grant objective was to test the hypothesis that the pro-inflammatory cytokine gamma interferon contributes to inflammation-associated neuropathology by acting directly on neurons to induce dendrite retraction and synapse loss.

R56 ES014901 (Lein) 07/01/07 – 06/30/08 NIH NIEHS
Molecular and Cellular Basis of PCB Developmental Neurotoxicity. The main grant objective was to test the hypothesis that non-coplanar PCBs disrupt neuronal connectivity via ryanodine receptor (RyR)-mediated mechanisms that modulate Ca2+-dependent signaling pathways linked to activity-dependent dendritic growth and plasticity.
Project #2007-27 (Lein and Tanguay) 01/01/06 – 02/01/08

Johns Hopkins University Center for Alternatives to Animal Testing (CAAT)
Zebrafish as an In Vivo Model System for Identifying Developmental Neurotoxicants. The overall goal of this project is to evaluate zebrafish as an alternative model for developmental neurotoxicity testing.

R21 NS45037 (Lein) 01/01/04 – 12/331/06
NIH NINDS
Molecular regulation of primary dendritogenesis. The main grant objective was to identify genes that control the growth of primary dendrites in mammalian neurons by using subtractive hybridization techniques to isolate genes that are differentially regulated in sympathetic neurons during BMP-induced dendritic growth.

MRF Seed grant (Lein) 12/01/05 – 11/30/06
Medical Research Foundation of Oregon
Mechanisms of organophosphate-induced neuronal M2 receptor dysfunction. The goal of this project is to address the question of how organophosphorus pesticides inhibit M2 receptors in parasympathetic nerves that innervate the airways.

R21 ES11771 (Lein) 04/30/02 – 07/31/05
NIH NIEHS
Mechanism of organophosphate developmental toxicity. The goal was to test the hypothesis that organophosphate insecticides disrupt axonal growth in the developing nervous system by interfering with the morphogenic activity of AChE.

PR012236 (Fryer) 01/31/02 – 03/31/05
United States Department of Defense
Mechanistic studies investigating the role of organophosphate insecticide exposure in the development and exacerbation of asthma. The goal was to test the hypothesis that organophosphate insecticides alter airway responsiveness by altering cholinergic neurotransmission in the lung. Role on grant: Co-Investigator

R03 HD40936 (Lein) 03/01/02 – 08/31/04
NIH NICHD
Do PCBs alter developing brain structure and cognition? The goal was to test the hypothesis that PCBs alter cognitive behavior in juveniles via effects on dendritic morphogenesis.

P30 ES03819 (Groopman) 04/01/01 – 03/31/08
NIH NIEHS
Johns Hopkins Center in Urban Environmental Health. The major goal of this project is to identify environmental chemical exposures and susceptibility factors that alone or together increase risk of disease for people living in urban environments. Role on grant: Director, Facility Core for Cell and Tissue Analysis, Apr 01 to Aug 03

T35 ES07308 (Lein) 04/01/03 – 03/31/04 NIH NIEHS
Short Term Research Training for Minority Students. The main objective was to provide opportunities for qualified undergraduate under-represented students to participate in research on the mechanisms of the adverse effects of environmental agents on human health.

Pilot grant (Lein) 05/01/01 – 04/30/02
The Thomas and Carol McCann Innovative Research Fund for Asthma and Respiratory Disease
Modulation of muscarinic receptor expression on sympathetic neurons by pro- and anti-inflammatory agents associated with asthma. The goal was to test the hypothesis that pro- and anti-inflammatory agents (IFN- and dexamethasone) modulate M2 receptor expression in sympathetic neurons.

Pilot project proposal 00-12 (Lein) 04/01/00 – 03/31/01
NIEHS, Johns Hopkins University Center in Urban Environmental Health
Effects of polychlorinated biphenyls on estrogen metabolism in the developing hippocampus . The goal was to test the hypothesis that perinatal exposure to PCBs causes neural deficits by altering estrogen metabolism in neural structures critical to cognitive function, such as the hippocampus.

Pilot project (Lein) 05/01/00 – 04/30/02
Johns Hopkins University Bloomberg School of Public Health
Regulation of dendritic growth by target-derived bone morphogenetic proteins. The specific aims of this pilot grant are to test the hypothesis that BMPs derived from target tissues regulate dendritic growth in sympathetic neurons. The long-term objective is to develop testable hypotheses regarding the molecular mechanism(s) by which environmental neurotoxins disrupt neuronal morphogenesis, specifically dendrite formation.

Pilot Project Proposal 99-12 (Lein) 07/01/99 – 06/30/00
NIEHS, Johns Hopkins University Center in Urban Environmental Health
Assessment of dendritic growth as a targeted process in the developmental neurotoxicity of polychlorinated biphenyls (PCBs). The hypothesis being tested in this pilot project is that PCBs alter dendritic growth in primary neuronal cultures.

AREA NS/OD36401 (Lein) 04/01/97 – 08/31/99
NIH NINDS
Regulation of dendritic growth by osteogenic protein-1. The goal was to characterize the effects of OP-1 on dendritic growth in cultured sympathetic neurons, and determine whether the spatial and temporal distribution of OP-1 and other BMPs in sympathetic ganglia and target tissues is consistent with their proposed role in regulating dendritic development in sympathetic neurons.

Pilot project (Lein) 01/01/91 – 12/31/91
American Cancer Society, Roswell Park Cancer Institute
Cell biology of human TILs engrafted into SCID mice. The goal was to compare the integrin expression profile on tumor infiltrating lymphocytes versus peripheral blood lymphocytes.

ACADEMIC SERVICE:

University of California at Davis

School of Veterinary Medicine

Member, Student Affairs Committee, 2010 – 2012
Member, Recruitment Committee for the Toxicology Faculty Position in the Davis California Animal Health and Food Safety Laboratory (CAHFS), 2009-2011.
Member, Curriculum Development Committee, Neurology/Senses/Behavior, 2009 - 2011
Member, Curriculum Development Committee, Pharm/Tox/Nutrition, 2009 - 2011

University

Member, Executive Committee, The Marion Miller Endowment Fund, 2011 - present
Member, Review Team, UC Davis Animal Biology Graduate Group Review, December 2011
Judge, UC Davis Interdisciplinary Graduate and Professional Student Symposium, 2011, 2012
Chair, Pharmacology and Toxicology (PTX) Graduate Group, 2010 – present
PI, NIEHS Training Grant, Advanced Training in Environmental Toxicology, 2010 - present
Member, Chemical and Laboratory Safety Committee, 2010 – present
Interviewer for student recruitment, Neuroscience Graduate Group, 2010, 2011
Member, Executive Committee, PTX Graduate Group, 2009 – 2010
Member, Admissions Committee, PTX Graduate Group, 2009, 2010
Fellowship Reviewer, Graduate Council, Support & Welfare Committee, 2009-2010
Member, Executive Committee, NIEHS Training Grant, Advanced Training in Environmental Toxicology, 2009 – present

Oregon Health & Sciences University

CROET

Chair, Faculty Budget Committee, 2008
Member, Search Committee for Faculty in Occupational Safety and Health, 2004, 2005

Program in Molecular and Cellular Biology (PMCB)

Member, Graduate Admissions Committee, 2004-2007
Member, PMCB Comprehensive Exam Committee, 2007 – 2008
Chair, PMCB Comprehensive Exam Committee, 2008-2009

University

Member, SOM Graduate Program Awards Committee, April 2006 – April 2009
Member, Institutional Animal Care and Use Committee, Nov 2005 – June 2008

Community

Judge, Intel International Science and Engineering Fair, Portland, OR, May 2004
Steering Committee Member, Workshop on the Medical Impacts of Childhood Hunger, Oregon Food Bank, Portland, OR, November 2004

Johns Hopkins University

Division and/or Department

Division Representative to Departmental Seminar Committee, 2003
Director, Minority Summer Internship in Environmental Health Sciences, 2003
Coordinator, Toxicological Sciences Comprehensive Preliminary Exam, 2002, 2003
Search Committee for Assistant Professor in Toxicological Sciences, 2001, 2002, 2003
Ad hoc Committee, Departmental Website Design, 2001 - 2002
Coordinator, Divisional Journal Club in Neurotoxicology, 2000 - 2001
United Way Solicitor, Environmental Health Sciences, 2000 - 2001

School

Reviewer, MPH Integrating Experience Projects 2000 - 2002
Director, Facility Core, Johns Hopkins Center in Urban Environmental Health, 2001-2003
Member, Johns Hopkins Center in Urban Environmental Health Research Council, 2002- 2003
Faculty Senator, 2002 - 2003

University

Voting Member, Institutional Animal Care and Use Committee, 2001 - 2003
Member, Rodent Advisory Committee, 2002 - 2003

Community

Judge, 8th Annual Undergraduate & Graduate Science Research Symposium Program, Morgan State University, Baltimore, MD, 1999, 2003
Speaker, "Women Serious about Science" program, Baltimore Polytechnic Institute, 2002

PRESENTATIONS

Scientific Meetings

Society of Toxicology, 2012

Banks CN, Zolkowska D, Dhir A, Inceoglu AB, Sanborn JR, McCoy MR, Bruun DA, Hammock BD, Rogawski MA, Lein PJ, Characterization of tetramethylenedisulfotetramine (TETS) toxicity in mice.
Barnhart CD, Yang D, Chen H, Truong K, Bose D, Pessah IN, Lein PJ, Developmental PCB 95 exposure interferes with spatial memory in weanling mice.
Bruun DA, Li Y, Ford BD, Lein PJ, Neuregulin-1 is neuroprotective against delayed neuronal injury following acute organophosphate intoxication.
Chen H, Bose DD, Maxwell SC, Lein PJ, Pessah IN, Ryanodine receptor-1 mutation potentiates PCB 95 effects on calcium oscillations in primary cortical neurons.
Korwel I, Barnhart CD, Truong KM, Lein PJ, Lehmler HJ, Enantioselective formation of hydroxylated metabolites of PCB95 in female mice.
Lein PJ, Wayman GA, Bose DD, Yang D, Lesiak A, Bruun D, Pessah IN, Ryanodine receptors are required for basal and PCB-modified activity-dependent dendritic growth.
Stamou M, Wu X, Korwel I, Lehmler HJ, Lein PJ, Cytochrome P450 enzyme expression in the rodent brain.
Yang D, Chen H, Mangini L, Pessah IN, Lein PJ, BDE-47 and BDE-49 influence morphologic determinants of neuronal connectivity in primary cultures of hippocampal neurons.

27th International Neurotoxicology Conference, 2012

VandeWater J and Lein PJ, Influence of the immune system on neurodevelopment.

5th Annual CounterACT Network Research Symposium, 2011

Lein PJ and Rogawski MA, Identification of novel therapeutic approaches for TETS and OP intoxication.

International Neurotoxicology Association (INA-13), 2011

Lein PJ, In vitro endpoints of relevance to organophosphorus pesticide-induced neurobehavioral deficits.

Society of Toxicology, 2011

Bruun D, Rowland D, Li Y, Ford B, Lein P. PET imaging of translocator protein 18 kDa (TSPO) reveals delayed neuroinflammation coincident with persistent behavioral deficits in rats acutely intoxicated with DFP.
Yang D, Kania-Korwel I, Bose D, Ghogha A, Pessah I, Lehmler HJ, Lein P. Enantioselective effects of PCB136 on dendritic growth are ryanodine receptor-dependent.
Bose DD, Yang D, Wayman GA, Lesiak A, Bruun DA, Pessah IN, Lein PJ. Non-dioxin-like polychlorinated biphenyls (PCBs) enhance dendritic growth in cultured hippocampal neurons via ryanodine receptor (RyR)-dependent activation of calcium-dependent signaling pathways.
Niknam Y, Ghogha A, Lein P, Pessah I. Chlorpyrifos and chlorpyrifos oxon alter ryanodine receptor function.
Anger WK, Farahat FM, Lein PJ, Olson JR, Rohlman DS. Behavioral deficits in Egyptian application teams with chronic organophosphorus pesticide exposures.
Ellison CA, Knaak JB, McDougall R, Lein PJ, Farahat FM, Anger WK, Olson JR. Construction and validation of a human PBPK/PD model for dermal chlorpyrifos exposure utilizing human biomarker data.
Hussainzada N, Jackson D, Bruun D, Milatovic D, Lewis J, Banks C, Aschner M, Browne R, Olson JR, Lein P. Biomarkers of neurotoxicity in a rat model of occupational chlorpyrifos (CPF) exposure.

Neurotoxicology 26, 2010

Bose DD, Kaplan ES, Lein PJ, Pessah IN. Non-coplanar PCBs increase spontaneous synchronized Ca2+ oscillations in primary hippocampal neurons.
Kim KH, Bose DD, Riehl J, Padilla IT, Barnhart CD, Lein PJ, Pessah IN. Para-substitution is a key determinant of brominated diphenyl ether activity towards ryanodine receptors.
Yang D and Lein P, Chlorpyrifos disrupts neuroligin-mediated synapse formation.

Society of Toxicology, 2010

Bose DD, Kaplan ES, Lein PJ, Pessah IN. Non-coplanar PCBs increase spontaneous synchronized Ca2+ oscillations in primary hippocampal neurons.
Crane AL, Browne RW, Knaak JB, Bonner MR, Fenske RA, Farahat FM, Anger WK, Lein PJ, Olson JR. Inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in human blood following in vitro and in vivo exposure to chlorpyrifos.
Crofton K, Buzanska L, Coecke S, Knaut H, Lein P, Mundy W, Price A, Seiler A, Goldberg A. Recommendations for developing alternative test methods for developmental neurotoxicity.
Ellison CA, Lein PJ, Knaak JB, Fenske RA, Farahat FM, Bonner MR, Anger WK, Olson JR. Exposure estimates and PBPK modeling of chlorpyrifos in rats and humans.
Farahat FM, Olson JR, McGarrigle BP, Bonner MR, Ellison CA, Fenske RA, Galvin K, Rohlman DR, Anger WK, Lein PJ. Biomarkers of chlorpyrifos exposure and effect in Egyptian cotton field workers.
Lein PJ, Farahat FM, Olson JR, Rohlman DR, Bonner MR, Lattal M, Fenske RA, Galvin K, Lasarev M, Anger WK. Biomarkers of Organophosphorus Pesticide-Induced Neurotoxicity.
Lattal M, Yang D, Bruun D, Anger WK, Lein PJ. Subchronic chlorpyrifos exposure alters appetitive behavior and contextual fear conditioning in rats.
Olson JR, Lasarev M, Bruun D, Milatovic D, Aschner M, Lein PJ. Strain- and dose-related effects of subchronic chlorpyrifos (CPF) exposure on biomarkers of exposure and oxidative stress in rats.
Yang D and Lein P, Chlorpyrifos disrupts neuroligin-mediated synapse formation.

World Congress 7, 2009

Lein PJ (Invited presentation). Overview of Developmental Neurotoxicity Testing (DNT): problems and approaches for minimizing animal use and maximizing data collection

Annual Force Health Protection Conference, 2009

Lein PJ (Invited presentation). Novel neuroprotectants in OP-induced neurotoxicity.

International Conference on Occupational Health, 2009

Anger WK, Rohlman DS and Lein PJ. Neurotoxicity of pesticides in old and young generations.

Annual CounterAct meting, 2009

Ford BD and Lein PJ. Neuroprotective role for neuregulins in neurotoxin-mediated neuronal injury.

Society of Toxicology, 2009

Barnhart C, Lauridsen H, Bruun D and Lein PJ. Neuregulin-1 protects against paraoxon-induced apoptosis in hippocampal slice cultures.
Courter LA, Gonsiorek EA, Garred M, Bruun D, Fryer AD, Higgins D and Lein PJ. Interferon-gamma causes dendrite retraction in sympathetic neurons in vivo.

DNT2 Workshop, 2008

Lein, PJ (Invited), Alternative systems-based models for developmental neurotoxicity testing.

Neurotoxicology 25, 2008

Ford BD, Lein PJ, Yonggang L and Bruun D (Invited), Neuregulin-1 protects against organophosphorus pesticide-induced neurotoxicity.
Yang D, Ladu J, Buels K, Lauridsen H, Tanguay R and Lein P, Chlorpyrifos-oxon disrupts motor behavior and axonal growth in embryonic zebrafish.
Courter LA, Garred M, Bruun D and Lein PJ, Interferon-gamma by peripheral inflammation causes dendritic retraction in distant neuronal loci.
Lein PJ (Invited), Using in vitro models to study gene-environment interactions in autism.

Pacific Northwest Association of Toxicologists (PANWAT) 25th Annual Meeting, 2008

Lein PJ (Invited presentation), Polychlorinated biphenyls (PCBs) modulate neuronal connectivity in the developing brain.

The Fifth PCB Workshop, 2008

Lein PJ (Invited presentation), Non-coplanar polychlorinated biphenyls (PCBs) modulate the development of neuronal connectivity via effects on the ryanodine receptor.

Society of Toxicology, 2008

Bruun D, Proskocil B, Fryer AD and Lein PJ, Organophosphorus pesticides (OPs) do not interact directly with airway nerves to cause neuronal M2 receptor dysfunction.
Ledoux VA, Wayman GA, Pessah IN, Lein PJ, Polychlorinated biphenyls (PCBs) influence dendritic growth in cultured hippocampal neurons via ryanodine receptor (RyR)-dependent activation of CaM kinase I.

Society of Toxicology, 2008, continued

Yang D, Ladu J, Buels K, Lauridsen H, Tanguay R and Lein PJ, Chlorpyrifos-oxon interferes with axonogenesis in embryonic zebrafish.

Neurotoxicology 24, 2007

Lein PJ (Invited presentation), Exposure of the developing brain to polychlorinated biphenyls influences susceptibility of the adult brain to stress.

2007 Dioxin Meetings, 2007

Lein PJ (Invited presentation), Ontogenetic alterations in molecular and structural correlates of dendritic growth following developmental exposure to polychlorinated biphenyls (PCBs).

The 33rd Annual Summer Meeting of The Toxicology Forum, 2007

Lein PJ (Invited presentation), Emerging models in developmental neurotoxicity testing: In vitro.
The 11th Meeting of the International Neurotoxicology Association, 2007
Lein PJ (Invited presentation), Polychlorinated biphenyls (PCBs) modulate the development of neuronal connectivity.

Society of Toxicology, 2007

Lein PJ (Invited presentation) Cell and molecular mechanisms of neurodevelopment: clues for identifying environmental risk factors for autism?
Yang D, Dziennis S, Alkayed N, Hurn P, Lein PJ, Developmental exposure to polychlorinated biphenyls reduces infarct size in the adult rat following ischemic stroke.

Society for Neuroscience, 2006

Lein PJ, D Yang, TR Soderling, IN Pessah and GA Wayman, Polychlorinated biphenyls enhance dendritic growth via ryanodine receptor-dependent Cam Kinase I activation and CREB-mediated transcription of Wnt.
Dziennis SE, D Yang, PJ Lein, NJ Alkayed and PD Hurn, Developmental exposure to polychlorinated biphenyls reduces infarct size in the adult rat following ischemic stroke.

Society of Toxicology, 2006

Lein PJ, D Yang, A Howard and D Bruun, Chlorpyrifos inhibits axon outgrowth via disruption of the morphogenic activity of acetylcholinesterase.
Yang D, KH Kim, A Phimister, IN Pessah, and PJ Lein, Developmental exposure to Aroclor 1254 impairs dendritic plasticity and functional expression of ryanodine receptors in weanling rats.

NIEHS, Genotype to Phenotype Correlations in Health and Disease, 2005

Lein PJ, D Yang, AS Howard and D Bruun, The acetylcholinesterase (AChE) knock-out mouse as a model for mechanistic studies of organophosphorus pesticide developmental neurotoxicity.

Society for Neuroscience, 2005

Lein PJ, X Guo, GX Shi, M Moholt-Siebert and DA Andres, The small GTPase Rit differentially regulates axonal and dendritic growth in sympathetic and hippocampal neurons.
Gonsiorek E, M Garred, D Higgins and PJ Lein, -Interferon (IFN) causes dendritic retraction in sympathetic neurons in vivo.
Gonsiorek EA, D Higgins and PJ Lein, Bone morphogenetic proteins regulate dendritic outgrowth in rat sympathetic neurons in vivo.

22nd International Neurotoxicology Conference, 2005

Lein P, E Gonsiorek, M Garred and D Higgins, Gamma-interferon causes dendritic retraction in sympathetic neurons in vivo.

Society of Toxicology, 2005

Yang D and P Lein, Altered cognitive function and dendritic growth in weanling rats exposed developmentally to Aroclor 1254 (abstract 1084).

Peer-Reviewed Medical Research Program (PRMRP) Military Health Research Forum, 2004

Fryer AD and P Lein, Mechanisms of organophosphate insecticide-induced airway hyperreactivity.

Society of Toxicology, 2004

Lein P and AD Fryer, Mechanisms of organophosphate insecticide-induced airway hyperreactivity.

21st International Neurotoxicology Conference, 2004

Howard AS and PJ Lein, Polychlorinated biphenyls induce caspase-dependent cell death in cultured embryonic rat hippocampal but not cortical neurons via activation of the ryanodine receptor.

International Neurotoxicology Association, 2003

Lein PJ, A Goldberg, P Locke and E Silbergeld, In vitro and alternative approaches to developmental neurotoxicity testing (DNT).

Society of Toxicology, 2003

Howard AS, R Bucelli, DA Jett and PJ Lein, Chlorpyrifos inhibits axon outgrowth in primary cultures of peripheral neurons through inhibition of the morphogenic activity of acetylcholinesterase.

American Society for Cell Biology, 2002

Chen HL, HN Beck, BJ Hoffer and PJ Lein, Regulation of dendritic growth by target-derived BMPs.

Society for Toxicology, 2002

Howard AS, PL Lein (2002). PCBs alter apoptosis in primary cultures of hippocampal but not cortical neurons.
Lein PJ, AS Howard, RA Schuh, R Bucelli, DA Jett (2002) Organophosphate pesticides differentially alter axonal and dendritic growth in cultured sympathetic neurons.

Society for Neuroscience, 2001

Beck HN, H-L Chen, P Lein (2001). Regulation of dendritic growth by target-derived BMPs.

CAAT's 20^th Anniversary Celebration, 2001

Lein P, RA Schuh, R Bucelli, D Jett (2001). Cultured sympathetic neurons as a model system for investigating the developmental neurotoxicity of organophosphate pesticides.

Society of Toxicology, 2001

Lein P, R Schuh, R Bucelli, D Jett (2001). Chlorpyrifos inhibits axonal outgrowth in sympathetic neurons at concentrations that have no effect on the enzymatic activity of acetylcholinesterase. The Toxicologist 60:243.

Alternative Toxicological Methods for the New Millennium: Science and Application, 2000

Lein, PJ, RA Schuh, R Bucelli, DA Jett (2000). Cultured sympathetic neurons as a model system for investigating the developmental neurotoxicity of organophosphate pesticides.

American Society of Neurochemistry, 1999

Lein, PJ, D Dorsaneo, H Nagasawa, PL Kaplan, D Higgins (1999). Target tissues influence dendritic growth in sympathetic neurons via osteogenic protein-1. J Neurochem. 72(Suppl):S35C.

First European Conference on Bone Morphogenetic Proteins, 1998

Charette, M, D Rueger, G Withers, G Banker, P Lein, D Higgins. Neurotrophic activities of bone morphogenetic proteins.

15th International Neurotoxicology Conference, Manganese, 1997

Lin, W, P Lein, D Higgins, JA Roth. Manganese-induced toxicity and process outgrowth in rat PC12 cells.

Society for Neuroscience, 1997

Gallagher PJ, PJ Lein, V Chandrasekaran, AM Hedges, D Rueger, D Higgins. Glia regulate dendritic growth in rat sympathetic neurons via bone morphogenetic proteins. Society for Neuroscience Abstr 23:885.
Dorsaneo D, AM Hedges, D Rueger, D Higgins, PJ Lein. Target tissues influence dendritic growth in sympathetic neurons via osteogenic protein-1 (OP-1). Society for Neuroscience Abstr 23:885.

American Society for Cell Biology, 1996

Gallagher PJ, J Amodeo, H Howie, JA Roth, PJ Lein. Integrins mediate manganese-induced neuronal differentiation in PC12 cells. Molec Biol Cell Abstr 21:97a.

American Society for Cell Biology, 1995

Chandrasekaran V, AM Hedges, D Rueger, PJ Lein. Glial induction of dendritic growth in rat sympathetic neurons involves osteogenic protein-1 (OP-1). Molec Biol Cell Abstr 6:99a.

Society of Toxicology, 1994

Craig DK, JS Davis, PW Hoffman, LG Lee, PJ Lein. Toxic chemical risk acceptance guidelines for use in DOE facilities. The Toxicologist 14:1151.

Society for Neuroscience, 1994

Lein PJ, M Johnson, X Guo, D Rueger, D Higgins. Osteogenic protein-1 (OP-1) induces dendritic growth in cultured sympathetic neurons. Society for Neuroscience Abstracts 20:680.

Invited Seminars

• June 2012, In vitro approaches for developmental neurotoxicity testing, 10th International Conference on Early Toxicity Screening, Seattle, WA
• May 2012, PCBs modulate neuronal connectivity via ryanodine receptor-mediated mechanisms. 7th PCB Workshop, Arcachon, France
• March 2012, Parallel animal and human research identify neurotoxic effects of occupational exposures to the organophosphorus pesticide chlorpyrifos. Neurotoxicological effects of exposure to organophosphate compounds symposium, 51st Annual Meeting of the Society of Toxicology, San Francisco, CA
• January 2012, Cell and Molecular Mechanisms of PCB Developmental Neurotoxicity, Departmental seminar series, Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA
• October 2011, Influence of the immune system on neurodevelopment, 27th International Neurotoxicology Conference, Research Triangle Park, NC
• June 2011, Identification of novel therapeutic approaches for TETS and OP intoxication, 5th Annual CounterACT Network Research Symposium, Washington, D.C.
• June 2011, In vitro endpoints of relevance to organophosphorus pesticide-induced neurobehavioral deficits, International Neurotoxicology Association, Xi’an, China
• April 2011, Atopic Status Determines Inflammatory Cell Mediators in Organophosphorus Pesticide-Induced Airway Hyperreactivity, Lung Research Day Symposium, University of California, Davis, CA
• March 2011, Novel Neuroprotectants in OP-Induced Neurotoxicity, National SAVMA (Student Chapter of the American Veterinary Medical Association) Symposium, University of California, Davis, CA
• February 2011, Polychlorinated Biphenyls (PCBs) Modulate Neuronal Connectivity via Ryanodine Receptor-Dependent Mechanisms, Research Seminar Series, Washington State University School of Veterinary Medicine, Pullman, WA
• January 2011, Organophosphorus Pesticides: Environmental Risk Factors for Autism Spectrum Disorders, Research Seminar Series, UC Davis M.I.N.D. Institute, Sacramento, CA
• November, 2010, Organophosphorus Pesticides and Neurodevelopmental Disorders, Pharmacology and Toxicology departmental seminar series, SUNY at Buffalo, Buffalo, NY
• May 2010, Cellular and molecular mechanisms of organophosphorus pesticide-induced airway hyperreactivity, Center for Comparative Respiratory Biology and Medicine, UC Davis, CA
• April, 2010, Cellular and molecular mechanisms of organophosphorus pesticide-induced airway hyperreactivity, Molecular Microbiology and Immunology seminar, UC Davis, CA
• March, 2010, In vitro and other methods for identifying developmental neurotoxicants, Human Health Hazard Indicators Workshop, California Environmental Protection Agency, Sacramento, CA
• January, 2010, Organophosphorus Pesticides and Neurodevelopmental Disorders, Molecular Pharmacology and Experimental Therapeutics seminar series, Mayo Clinic College of Medicine, Rochester, MN
• December, 2009, The yin and yang of dendritic growth: bone morphogenetic proteins and proinflammatory cytokines, Center for Neuroscience Research at the Children's National Medical Center, Washington, D.C.
• November, 2009, In vitro toxicity testing: perspective of an academic scientist, Toxicity Testing in the 21st Century: Can We Make the Business Case for Alternatives? University of Chicago Law School, Chicago, IL
• November, 2009, Interference of neuronal morphogenesis by organophosphorus pesticides: potential relevance to autism spectrum disorders, Interdisciplinary Faculty of Toxicology seminar series, Texas A&M, College Station, TX
• October, 2009, Regulation of dendritic growth by cytokines, Neuroscience & Physiology Program, SUNY at Syracuse, Syracuse, NY
• September, 2009, Overview of Developmental Neurotoxicity Testing (DNT): problems and approaches for minimizing animal use and maximizing data collection, World Congress 7, Rome Italy.
• August, 2009, Novel neuroprotectants in OP-induced neurotoxicity. Annual Force Health Protection Conference, Albuquerque, NM.
• March 2009, Developmental neurotoxicity of organophosphorus pesticides: neuronal morphogenesis as a target, Integrated Toxicology and Environmental Health Program, Duke University, Durham, NC
• March 2009, Polychlorinated biphenyls (PCBs) modulate neuronal connectivity, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN
• November 2008, Alternative systems-based models for developmental neurotoxicity testing, DNT2 Workshop, Reston, VA
• October 2008, Metabolism and oxidative stress as modulators and mediators of PCB developmental neurotoxicity, Human Toxicology and EHSRC Research Seminar, University of Iowa, Iowa City, IA
• October 2008, Using in vitro models to study gene-environment interactions in autism, 25th Neurotoxicology Conference, Rochester, NY.
• September 2008, Polychlorinated biphenyls (PCBs) modulate neuronal connectivity in the developing brain, Pacific Northwest Association of Toxicologists (PANWAT) 25th Annual Meeting, Corvallis, OR
• May 2008, Non-coplanar polychlorinated biphenyls (PCBs) modulate the development of neuronal connectivity via effects on the ryanodine receptor, The Fifth PCB Workshop, Iowa City, IA
• January 2008, Control of dendritic growth by growth factors and cytokines, Neurological Sciences Institute, Oregon Health & Science University, Portland, OR
• January 2008, Alternatives to developmental neurotoxicity testing, Alternative Models for Animal Research, UCLA, Los Angeles, CA
• January 2008, PMCB Research Colloquium, Program in Molecular and Cellular Biology, Oregon Health & Science University, Portland, OR
• December 2007, Potential cell-based models for detecting endocrine disruption in the developing brain. GE Program on Alternative Methods, Center for Alternatives to Animal Testing, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD
• December 2007, Early brain development and environmental exposures. Children’s Development and Rehabilitation Center (CDRC) Grand Rounds, Oregon Health & Science University, Portland, OR
• November 2007, Exposure of the developing brain to polychlorinated biphenyls influences susceptibility of the adult brain to stress. Neurotoxicology 24, San Antonio, TX
• September 2007, Ontogenetic alterations in molecular and structural correlate of dendritic growth following developmental exposure to polychlorinated biphenyls (PCBs). Dioxin 2007, Tokyo, Japan.
• July 2007, Emerging models in developmental neurotoxicity testing: In vitro. The 33rd Annual Summer Meeting of the Toxicology Forum, Aspen, CO
• June 2007, Polychlorinated biphenyls (PCBs) modulate the development of neuronal connectivity. Workshop on Immunologic and Neurodevelopmental Susceptibilities in Autism, The 11th Meeting of the International Neuroscience Association (INA-11), Pacific Grove, CA
• March 2007, Cell and molecular mechanisms of neurodevelopment: clues for identifying environmental risk factors for autism? Workshop on Environmental Risk Factors for Autism, Annual Meeting of the Society of Toxicology, Charlotte, NC
• January 2007, In vitro approaches to developmental neurotoxicity testing (DNT), The First Indian Congress on Alternatives to the Use of Animals in Research, Testing and Education, Sri Ramachandra Medical College & Research Institute (Deemed University), Chennai, India
• November 2006, Control of dendritic growth by growth factors and cytokines, Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI
• March 2006, Neuronal morphogenesis as an endpoint for in vitro developmental neurotoxicity testing, TestSmart DNT (sponsored by the Johns Hopkins University Center for Alternatives to Animal Testing, USEPA, NIEHS, Rohm and Haas, RIFM, Shell Oil), Reston, VA
• January 2006, Organophosphate-induced delayed neuropathy, Neurology Grand Rounds, Oregon Health & Science University, Portland, OR
• November 2005, Interferon-gamma and dendrite retraction, Department of Anesthesiology, Oregon Health & Science University, Portland, OR
• October 2005, Cytokine control of dendritic growth, Cell and Developmental Biology, Oregon Health & Science University, Portland, OR
• October 2004, Mechanisms of Organophosphate Developmental Neurotoxicity, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR
• August 2004, Keynote Address: Physiological Evidence of a Link Between Organophosphorus Insecticides and Asthma, Sixth Annual UC Davis Conference for Environmental Health Scientists, Napa, California.
• March 2004, Mechanism of Organophosphate Developmental Neurotoxicity, Department of Pharmacology and Toxicology, SUNY at Buffalo School of Medicine and Biomedical Sciences, Buffalo, NY
• March 2004, Mechanism of Organophosphate Developmental Neurotoxicity, Joint NIEHS/ACC Developmental Toxicology RFA and Fetal Basis of Adult Disease PAR Grantee Meeting, Research Triangle Park, North Carolina
• January 2004, Cell and Molecular Mechanisms of Developmental Neurotoxicity, Department of Environmental and Biomolecular Systems, OGI School of Science & Engineering, Oregon Health & Science University, Portland, OR
• December 2003, Regulation of Neuronal Morphogenesis, Cell and Developmental Biology, Oregon Health & Science University, Portland, OR
• October 2003, Modulation of Dendritic Growth by Bone Morphogenetic Proteins, Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan, ROC
• June 2003, In Vitro and Alternative Approaches to Developmental Neurotoxicity Testing, The 9th meeting of the International Neurotoxicology Association, Dresden, Germany
• February 2003, The case of chlorpyrifos: AChE inhibition and beyond, Toxic Damage to Developmental Signals Symposium, Duke University Integrated Toxicology Program, Duke University, Durham, NC
• January 2003, Regulation of dendritic growth by BMPs, Biochemistry and Molecular Biology Open Seminar Series, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD
• November 2002, Non-cholinergic mechanisms of organophosphate developmental neurotoxicity, CROET, Oregon Health Sciences University, Portland, OR
• April 2002, BMPs in regulation of dendritic growth, CROET, Oregon Health Sciences University, Portland, OR
• March 2002, Regulation of dendritic growth by bone morphogenetic proteins, Biology Dept, Indiana University Purdue University at Indianapolis (IUPUI), Indianapolis, IN
• February 2002, Extrinsic factors that influence neuronal morphogenesis, USEPA, Neurotoxicology Division, Research Triangle Park, NC
• January 2002, Regulation of dendritic growth by bone morphogenetic proteins (BMPs), Kennedy Krieger Institute, Baltimore, MD
• August 2001, Mechanisms by which endocrine disruptors disrupt neuronal development in mammalian systems, EDICOR Site Visit, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD
• July 2001, Regulation of dendritic growth by bone morphogenetic proteins (BMPs), Dept Anatomy and Neurobiology, University of Kentucky School of Medicine, Lexington, KY
• December 2000, Assessment of Dendritic Growth as a Targeted Process in the Developmental Neurotoxicity of PCBs. Johns Hopkins NIEHS Center in Urban Environmental Health, External Advisory Committee Meeting, Johns Hopkins University, Baltimore, MD
• November 2000, Regulation of Dendritic Growth by Bone Morphogenetic Proteins (BMPs). Biomedical Research Seminars Series, Morgan State University, Baltimore, MD
• April 2000, Environmental Toxins: Hackers that Target the Hardware of the Neural Network, Environmental Health Science Faculty Meeting with Dean Sommer, Johns Hopkins University School of Public Health, Baltimore, MD
• April 2000, Positive and negative regulation of dendritic growth, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, Washington, DC
• February 2000, Regulation of dendritic growth in sympathetic neurons, Department of Molecular Microbiology and Immunology, Neurovirology, Johns Hopkins University School of Public Health, Baltimore, MD
• October 1999, Cell and molecular mechanisms underlying dendritic growth in sympathetic neurons, Department of Environmental Health Sciences, Division of Physiology, Johns Hopkins University School of Public Health, Baltimore, MD
• November 1997, Regulation of neuronal morphogenesis by osteogenic protein-1, Alpha Theta chapter of Tri Beta, Canisius College, Buffalo, NY
• October 1997, Regulation of neuronal morphogenesis by osteogenic protein-1, Biochemical Pharmacology, SUNY at Buffalo, School of Pharmacy, Buffalo, NY
• May 1997, Bone morphogenetic proteins (BMPs) regulate neuronal differentiation, Toxicology Research Center, Environmental Health Sciences Graduate Group, SUNY at Buffalo School of Medicine and Biomedical Sciences, Buffalo, NY
• March 1997, Adhesion molecules: more than cellular glue, Department of Biology, Niagara University, Niagara Falls, NY
• November 1996, The human genome project, American Council on Education, National Identification Program, Canisius College, Buffalo, NY
• November 1996, Bone morphogenetic proteins (BMPs) regulate neuronal differentiation, Alpha Theta chapter of Tri Beta, Canisius College, Buffalo, NY
• January 1996, Osteogenic protein-1 specifically induces dendritic growth in sympathetic neurons in culture, Lung Biology Research Program, Buffalo General Hospital, Buffalo, NY
• September 1995, Regulation of dendritic growth in sympathetic neurons, Departments of Psychology and Biology, Hamilton College, Clinton, NY
• August 1995, Environmental specification of neuronal morphology: role of BMP-7 in dendritic growth, Dept Neurology, University of Tubingen, Tubingen, Germany
• June 1994, Extracellular matrix molecules as determinants of neuronal morphology, Lung Biology Research Program, Buffalo General Hospital, Buffalo, NY

ADDITIONAL INFORMATION

Personal statement of research and research objectives

The long-term goal of my research program is to identify and characterize environmental stressors that cause adverse neurodevelopmental outcome and to examine how such stressors interact with genetic susceptibilities to determine risk and outcome severity of neurodevelopmental and neurodegenerative diseases. Altered patterns of connectivity are associated with most neurodevelopmental diseases, and often present as early pathological findings in neurodegenerative diseases. Therefore, we are currently investigating how organic pollutants (PCBs and PBDEs), air pollutants (particularly PAHs), pesticides (organophosphorus pesticides [OPs], carbamates and pyrethroids) and inflammation perturb neuronal connectivity. In addition to studies focused on the cellular and molecular mechanisms by which these environmental stressors interfere with neuronal connectivity as determined using biochemical, morphogenic and electrophysiological endpoints, we are also developing biomarkers of OP neurotoxicity and testing novel therapeutic approaches for protecting against the neurodegenerative effects associated with neurotoxic pro-convulsants, specifically OPs and tetramines. We are investigating these questions using histological, behavioral and molecular techniques in both in vivo (guinea pigs, rats and transgenic mice) and in vitro (primary neuronal cultures) model systems.