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Johns Hopkins Bloomberg School of Public HealthCAAT

Refinement Program

Kathrin Herrmann

Refinement on the Way Towards Replacement: CAAT's Evidence-based Refinement Program

Coordinator: Kathrin Herrmann (

“Suppose, for a particular purpose, we cannot use replacing techniques. Suppose it is agreed that we shall be using every device of theory and practice to reduce to a minimum the number of animals we have to employ. It is at this point that refinement starts, and its object is simply to reduce to an absolute minimum the amount of distress imposed on those animals that are still used.”
—The Principles of Human Experimental Technique,
(Russell and Burch,1959, Chapter 7) [emphasis added] 

Russell and Burch introduced the principles of replacement, reduction, and refinement of animal experimentation in 1959 in their groundbreaking book, The Principles of Humane Experimental Technique (Russell and Burch, 1959)to eradicate inhumanity towards other animals used in science. Their highest goal was to avoid the use of animals wherever possible, and—in the case that animals were still deemed indispensable—to enhance significantly their treatment, while also improving the quality of research and testing. Thus, CAAT’s Refinement Program includes not only environmental (housing) and experimental refinements in its agenda, but also a strong focus on refinement of planning, conducting, and reporting of animal-based studies, as these are essential to ensuring the quality of research and testing. A new priority of CAAT’s Refinement Program is to help fund systematic reviews of frequently used and/or severe animal models to either refine them (if proven valid) or, when they are not representative for the intended purpose (e.g., to model a specific human malady), to demonstrate their weaknesses in an unbiased manner.

Through our next generation Science-Based Refinement Awards, we provide funding for non-invasive research that helps to improve both animal welfare and scientific rigor. These awards focus on research projects to enhance the housing, handling, and/or experimental situations for laboratory animals or reduce animal use (by, for example, identifying areas of research and testing where animals are not suitable). The grants are intended for those who work hands-on with animals, such as animal welfare scientists, veterinarians, and animal care technicians, as well as for researchers who conduct systematic reviews and meta-analyses in all the above-mentioned areas of refinement. These awards have an impact well beyond the recipients themselves; past awardees have been able to publish their results in international journals, and have received grants from other organizations to continue their work, thereby effecting long-lasting impacts on animal lives. 

Science-based Refinement Awards (Full information and Call for Proposals)

In addition to the awards, the Refinement Program is engaging in the following activities: 

  • Teaching at Johns Hopkins University and for FELASA courses in Europe (e-learning), as well as speaking at international conferences
  • Organizing international symposia, workshops, and seminars on pressing refinement issues
  • Co-organizing an annual symposium on social housing and other refinement in the US. Presentations from our 2018 Symposium on Social Housing of Laboratory Animals can be viewed here
  • Researching ways to improve scientific rigor and reproducibility 

2018 Activities

Presentations at international conferences:

September 23-26, 2018 
European Society for Alternatives to Animal Testing (EUSAAT), 18thAnnual Congressin Linz, Austria

September 27-29, 2018: 
European Veterinary Congress of Behavioural Medicine and Animal Welfarein Berlin, Germany

October 15-16, 2018: 
ESLAV, ECLAM, AAALAC International and SECAL Conferencein Barcelona, Spain

Co-organization and sponsorship of a seminar at 69thAALAS National Meeting in Baltimore, MD, USA:
November 1, 2018:

The Laboratory Animal Medical Professional's Guide to How the 3Rs Enhance Rigor and Reproducibility
8:00 AM - 10:15 AM/Room: Ballroom III
Leaders: Kathrin Herrmann, Eric K Hutchinson, Kelly A Metcalf Pate
Moderator: Casey Kissel
Facilitator: Adam D Werts

Animal research has come under increased scrutiny recently due to a perceived lack of rigor and reproducibility. The laboratory animal research community has a unique responsibility to ensure the validity of animal research studies while also caring for the well-being of the research subjects. This highly interactive seminar will focus on the importance of preparation, information gathering, housing and husbandry, analgesia and anesthesia, and reporting to assure high-quality, translational science. We will start with a discussion of the necessary steps to ensure effective research before a study even begins, and why pre-registering animal studies ensures better quality research outcomes. We will demonstrate and practice strategies for effectively searching existing literature and reports. We will make the connection between different housing, husbandry, analgesia, and anesthetic practices and rigorous science. We will lay out the importance of data-sharing and full reporting beyond the typical methods and results to the reproducibility of animal experiments. Throughout the seminar, attendees will have the opportunity to apply the concepts we present to real-world examples of animal research proposals. This seminar series is suitable for all laboratory animal professionals and will give participants a strong understanding of how the 3Rs impact rigorous and reproducible science.

Science Based-Refinement Awards
Deadline for receipt of submissions is December 31, 2018
Call for Proposals


June 3-4, 2019
2019 Symposium on Social Housing of Laboratory Animals
USDA National Agricultural Library
Beltsville, MD
In collaboration with USDA Animal Welfare Information Center, NIH Office of Laboratory Animal Welfare, and the Johns Hopkins Department of Molecular and Comparative Pathobiology.