The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1986-1987

Surfactant Induced Primary Irritancy -- An In Vitro Model

Vincent A. De Leo, MD
University College of Physicians and Surgeons, New York City, New York

DeLeo has already demonstrated that skin irritants cause the release of two biological molecules, choline and arachidonic acid, from animal and human skin cells in vitro. He will investigate the role of these molecules in the irritation process. He also will try to develop an in vitro alternative to the Draize skin test based on measurement of arachidonic acid release from human skin cells called kerotinocytes.