The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1987-1988

Establishment of Differentiated Human Cell Lines by Transfection with Oncogenes

Orest Hurko, MD
The Johns Hopkins Hospital, Baltimore, Maryland

Hurko has successfully grown cells in culture from humans with inheritable muscle disease by adding oncogenes. These cells proliferate rapidly and still can differentiate. With his CAAT grant, he will determine the stability of the cell lines and establsh methods to maintain the cells' ability to differentiate. Hurko's cell lines, once established, would prove useful in understanding muscle disease and in developing cell lines from other types of human tissue.