The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1990-1991

An In Vitro Bioassay for Antiprogesterone Activity Associated with Early Abortion

Robert M. Bigsby, PhD
Indiana University, Indianapolis, Indiana

Bigsby hopes to construct an in vitro bioassay for progesterone action, useful in screening potential progesterone antagonists which can induce abortion during early pregnancy. He has found that synthesis of a characteristic progesterone induced protein (PIP) is stimulated by progesterone and inhibited by progesterone antagonists. Bigsby is developing a radioimmunoassay for PIP to measure presence of progesterone antagonists in endometrial cell cultures.