The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.
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Research Grants 1990-1991
Enhancing Smooth Muscle Properties of Cultured Rat Myometrium
J. Frederick Krall, PhD
VA Medical Center, Sepulveda, California
To evaluate agents involved in increased uterine motility and, hence, spontaneous abortions, Krall is developing cultures of rat uterine myometrial cells. Because such cultures often lose their natural contractile properties and ability to mobilize calcium ions, Krall hopes to define conditions that will restore them. He also will note effects of ethanol versus acetaldehyde on the contractility of his cultured cells, monitoring that effect by appearance of a metabolite, inositol polyphosphate.