The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1993-1994

Development of Differentiated Sertoli Cell Lines for Testing Male Reproductive Toxicity

Kim Boekelheide, MD, PhD
Brown University, Providence, Rhode Island

In this second year grant, Boekelheide and colleagues continue their development of a Sertoli cell line for in vitro evaluation of testicular toxicants. The rat-derived cell lines will be screened by morphology, cytochemistry, immunocytochemistry and by secretion of the Sertoli cell protein, transferrin. Clones which express the most differentiated phenotype will be evaluated for various media supplements and culture substrates. The cell lines will then be evaluated previously validated parameters of Sertoli cell toxicity.