The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1993-1994

The Development of Immortalized Rabbit Kidney Proximal Tubule Cell Culture System in Serum Free Medium

Mary L. Taub, PhD
State university of New York at Buffalo, Buffalo, New York

Taub and colleagues are developing an immortalized rabbit kidney proximal tubule cell culture system for nephrotoxicity studies. The investigators have transformed primary rabbit kidney proximal tubule cells with pRSVT plasmid containing SV40 genes and have maintained the transformants in culture for over 20 passages.

They will now examine the cytotoxic effects of nephrotoxins on pRSVT transformed rabbit kidney cells and attempt to identify the mechanisms by which the nephrotoxins mercuric chloride and cephaloridine exert their cytotoxic effects. Glutathione levels, oxidative metabolism and the activity of the p-aminohippurate transport system will be examined in the pRSVT transformed cells following treatment with the toxins.