The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1993-1994

Biochemical Characterization of ML-1 Cell Differentiation for Toxicological Studies

Michael A. Trush, PhD
Johns Hopkins School of Public Health, Baltimore, Maryland

Trush and colleagues will use the human myeloblastic leukemia cell, ML-1, as a model of monocytic differentiation to evaluate the effects of xenobiotics on cell differentiation. They will develop a battery of assays which assess cell function and biochemistry and investigate the manner in which culture conditions may alter the differentiation and biochemistry of these cells

During the first year of the grant they plan to complete characterization of the ML-1 cell line, to validate the role of mitochondrial development in mononuclear cell function, to study the effects of modification of culture conditions and to examine the effect of the addition of cytokines on cell differentiation. They will also study the effects of hydroquinone on ML-1 cell differentiation and function.