The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1995-1996

Development of an In Vitro Model for Human Response to Dioxin

Thomas R. Sutter, PhD
Johns Hopkins School of Public Health, Baltimore, Maryland

In this thrid year grant, Sutter and colleagues continue development of an epithelial cell culture system for measurements of human response to TCDD (dioxin). Previous studies have led to the discovery of a growth factor autocrine loop that significantly alters the cellular dose-response of human epidermal keratinocytes (HEKs) to TCDD. Specific objectives at this stage of the project include assessment of the TCDD concentration-response relationships for decreased epidermal growth factor receptor (EGF-R) binding and cellular differentiation in a single strain of HEKs and determiniation of TCDD concentration-response relationships for multiple strains of HEKs.