The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1995-1996

Biochemical Characterization of ML-1 Cell Differentiation for Toxicological Studies

Michael A. Trush, PhD
Johns Hopkins School of Public Health, Baltimore, Maryland

The primary objective of Trush and colleagues is to use the human myeloblastic leukemia cell line, ML-1, as a model to evaluate the effects of xenobiotics on bone marrow myeloid stem cells and on monocytic differentiation. The specific aims of this third year grant are to examine the roles of tumor necrosis factor (TNF) and transferrin in the development of mitochondrial and NADPH oxidase activity, to examine the regulation of glutathione and quinone reductase during mononuclear cell differentiation, to examine the effects of lead on ML-1 cell differentiation and to use ML-1 cells to study the effects of benzo(a)pyrene-derived quinones on mitochondrial respiration and monocyte function. The lone term objective of the work is to extend the concepts learned with this myeloid cell system to other cell differentiation models of toxicological relevance and concern.