The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1995-1996

Role of Epithelium-Derived Cytokines in Oxidant-Induced Inflammation

Marsha Wills-Karp, PhD
Johns Hopkins School of Public Health, Baltimore, Maryland

Wills-Karp and colleagues hypothesize that ozone-exposed epithelial cells regulate the inflammatory response to ozone by releasing cytokines which activate leukocytes. Specifically, they will determine the optimal conditions for exposing BEAS-2B cells to ozone in vitro by conducting and characterizing air and ozone exposure chambers, determine the profile of proinflammatory cytokines produced by BEAS-2B cells following ozone exposure in vitro, and determine the role of epithelial-derived cytokines in the activation of leukocytes following ozone exposure by examining the effects of culture supernatants from ozone-exposed BEAS-2B cells on leukocyte activation and cytokine production.