The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.

 

Johns Hopkins School of Public Health

Research Grants 1996-1997

Fluorescent Probing of Oxidative Stress and Antioxidant Efficacy in a Cell Culture Model

Valerian E. Kagan, PhD
University of Pittsburgh, Pittsburgh, Pennsylvania

Extensive evidence implicates oxygen and lipid free radicals in a host of degenerative diseases and acute conditions. Lipid peroxidation is considered one of the major contributors to oxidative injury; inhibition of lipid peroxidation is extremely important in maintaining good health. The model being developed by the investigator will probe oxidative stress induced by chemicals and test antioxidants in biochemical models with in vivo-like metabolic pathways of activation and/or repair. Specific aims for the first year include optimization of the cell culture model for fluorescent probing of oxidative stress, validation of the model as a predictor of oxidative stress of chemicals, and validation of the model as a predictor of protective effects of antioxidant compounds.