The Center for Alternatives to Animal Testing is an academic center affiliated with the Division of Toxicological Sciences in the Department of Environmental Health Sciences of the Johns Hopkins University Bloomberg School of Public Health.
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TestSmart HPV
April 26-27, 1999
Hyatt Fair Lakes
12777 Fair Lakes Circle
Fairfax, VA 22033
A workshop of The Johns Hopkins Center for Alternatives to Animal Testing
TestSmart is a program of the Vision 20/20 forum
This workshop is partially funded through a grant by the Vira I. Heinz Endowment
Abstract for TestSmart--A Humane and Efficient Approach to Screening Information Data Sets (SIDS) Data
Seroli Cells, Early Germ Cells
Kim Boekelheide
Brown University
In Vitro Approaches: Toxicants and Seminiferous Epithelium
- Cell Culture
- Methods: immortalized cell lines, primary cells, co-culture of primary cells
- Uses: screening by "pop-off," mechanistic investigations
- Tubule culture
- Methods: short-term assays, culture, human
- Uses: toxicant exposure, seminiferous tubule fluid secretion, sperm release
Sertoi-Germ Cell Co-Cultures as a Model System
Table 2. Effect of di-(2-ethylhexyl) phthalate (DEHP) and some of its metabolites on germ cell detachment in Sertoli-germ cell co-cultures
| No. of germ cells detached (% of control) | ||||||
| Treatmenta | Test concn(µM) | 1 | 10 | 100 | 200 | 1000 |
| DEHP | - | - | - | 111 | - | |
| 2-Ethylhexanol | - | - | - | 102 | - | |
| MEHP | 126 | 141* | 198** | 258*** | Toxicb | |
| Metabolite Vc | - | 89 | 103 | - | 103 | |
| Metabolite VIc | - | 86 | 112 | - | 96 | |
| Metabolite XIc | - | 85 | 95 | - | 136* | |
MEHP = Mono-(2-ethylhexyl) phthalate
aFor 24 hours at an incubation temperature of 32°C.
bAt this concentration MEHP caused such extensive destruction of the cultures that cell counts were not possible.
cThe MEHP metabolites are numbered according to the scheme of Albro et al. (1982): V - mono (5-carboxy-2-ethylpentyl) phthalate (5-carboxyMEHP); VI - mono-(2-ethyl-5-oxohexyl) phthalate (5-oxoMEHP); IX - mono-(2-ethyl-5-hydroxyhexyl) phthalate (5-hydroxyMEHP).
Asterisked figures differ significantly from the control (by Student's T test): *P < 0.05; **P < 0.01; *** < 0.001.
T.J.B. Gray (1986) Fd. Chem. Toxic. 24:601-605